John Murray (University of Pennsylvania), Ke Hu (Indiana University, Bloomington) (2011) CIL:10552, Toxoplasma gondii RH. CIL. Dataset

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged Tgalpha1tubulin and mRFP-tagged TgDLC (dynein light chain). Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X, 1.4 NA. Filters: EGFP, ex 450-490 em 500-540, mRFP, ex 555-592 em 600-665. Dichromatic mirror: Chroma 89021. Captured with CoolSnapHQ/ICX285. Deconvolved data stack corresponds to raw data stack:CIL# 10551 and DIC image: CIL# 10553.

Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

一层人类表皮成纤维细胞被感染了表达YFP标记的Tgalpha1tubulin和mRFP标记的TgDLC（动力蛋白轻链）的弓形虫。利用Olympus IX70倒置显微镜搭载API DeltaVision系统采集图像。成像参数：目镜Olympus 60X，数值孔径1.4。滤光片：EGFP，激发波长450-490nm，发射波长500-540nm；mRFP，激发波长555-592nm，发射波长600-665nm。双色镜：Chroma 89021。图像采集设备为CoolSnapHQ/ICX285。去卷积数据堆栈对应于原始数据堆栈：CIL# 10551和DIC图像：CIL# 10553。此图像为未发表图像，与PMID: 16518471发表的图像相似。有关其他实验细节，请参阅相关出版物。