Evading immune evasion via reprograming of the tumor microenvironment by radiotherapy and simultaneous targeting of TGF-ß/PD-L1

Single cell transcriptome showed TGF-ß expression is confined to PD-L1+ endothelium and M2 /lipofibroblast-like cells. Hence, superior effects of BA could be attributed to its ability to trap TGF-ß to relevant PD-L1+ compartments. Mice were irradiated (photon) and one group of mice was treated with Bintrafusp. Lungs were subsequently dissociated and processed with the 10x genomics 3 prime v2 kit.