Cytotoxic effect of vital dyes on retinal pigment epithelial cells determined by LDH assay.

Analysis of variance (ANOVA) –Tukey test Observations:
I – Higher the values higher the dye toxicity.
II – Values are means of at least 3 independent experiments.
III – In regard to the P values presented in this table, there was a significant difference between the dyes with all concentrations and exposure times (* P<0.05, ** P<0.01) when compared with non-treated control cells.