Intrinsic toxicity of LCDD monoclonal Ig free light chain on plasma cells (PC)

We analyzed the transcriptome (RNA-seq) of mouse plasma cells (PC) expressing a human Ig light chain from a patient suffering light chain deposition disease (LCDD) as compared to control plasma cells (WT and DH-LMP2A, the latter producing no complete immunoglobulins, only free Ig light chains). Ig light chains causing LCDD present structural peculiarities leading to their aggregation and deposition into tissues and organs, mainly the kidney. We sought to determine if plasma cells producing these abnormal Ig could present specific phenotypes. The aim of the present study is to analyse the transcriptomic signature of plasma cells producing abnormal Ig free light chains. Spleen plasma cells from non-immunized mice were magnetically-purified using CD138 beads (purity > 80%). Each sample corresponds to the total CD138+ plasma cells from one spleen. Cells are from 3-month-old mice.