Prevotella intermedia culture supernatant impairs alveolar macrophage function and promotes Mycobacterium avium persistence

In NTM-PD, P.int sup. may modulate the functions of alveolar macrophages by suppressing autophagy, type 1 interferon responses, and anti-mycobacterial protein production, thereby promoting M. avium survival in the lung microenvironment Overall design: In vitro, total RNA was extracted from the AMLCs, which were infected with M. avium for 4 h, followed by treatment with P. int. sup. (or GAM broth) for an additional 4 h.