a, random mutagenesis; b, site-directed mutagenesis. Underline letters indicate the sequence for ligation-independent cloning; Italic letters indicate the mutation nucleotides. The reverse primers not

NIAID Data Ecosystem50

Oligonucleotide sequences used for cloning Ae. aegypti P450 genes.

基因编辑实验

昆虫抗药性

Oligonucleotide sequences used for cloning Ae. aegypti P450 genes.

Figshare2015-12-02 更新30

Primer sequences for TALE repeats amplification.

基因编辑工具

分子克隆引物设计

Primer sequences for TALE repeats amplification.

NIAID Data Ecosystem50

Table 2. Primers used to construct B19 VP1u point mutations and truncated VP1u.

病毒蛋白工程

分子克隆引物设计

aAll sequences are based on the sequence of the J35 isolate (GenBank accession no. AY386330).bGGATCC is a restriction site of BamHI, AAGCTT is a restriction site of HindIII.

Figshare2015-12-02 更新40

Primers.

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