Biological nitrogen fixation maintains C/N balance and photosynthesis at elevated CO2

To investigate the effect that biological nitrogen fixation will have on plant responses to nitrogen dose at elevated CO2, alfalfa (Medicago sativa) lines were grown at three nitrogen doses and ambient or elevated CO2. Four lines were used in the study, two lines that can form nodules capable of fixing nitrogen (effective lines) and two lines that can not form nodules capable of nitrogen fixation (ineffective lines). The ineffective lines are the result of a complementary mutation in the same gene. Overall design: RNA-seq

本研究旨在探究生物固氮对植物在高浓度二氧化碳环境下对氮肥剂量响应的影响。为此，选取了苜蓿（Medicago sativa）的三个氮肥剂量处理组，并在常温和高浓度二氧化碳环境下进行培养。研究中共使用了四条苜蓿品种，其中两条品种能够形成具有固氮能力的根瘤（有效品种），而另外两条品种则不能形成具有固氮能力的根瘤（无效品种）。无效品种的产生是由于同一基因上的互补突变所致。总体实验设计为RNA测序。