Small RNA-sequencing in primary breast fibroblasts treated and not-treated with breast cancer exosomes

We report the application of small-RNA-sequencing technology for high-throughput profiling of microRNAs in primary mammalian fibroblasts upon breast cancer-derived exosome treatment. We generated microRNA expression profile by obtaining 928(?) microRNA sequences diffrentially expressed between fibroblasts incubated with exosomes and with PBS (NT,negative control). Among 14 (?) microRNAs significantly upregulated in primary fibroblasts after exosome treatment, we demonstrated that microRNA-185-5p, microRNA-652-5p, and microRNA-1246 were actively involved in fibroblasts functional activation, thus corroborating our initial hypothesis. Examination of miRNAs differentially expressed in fibroblasts treated with breast cancer exosomes compared to not-treated fibroblasts