Expression data in EC109 and KYSE510 cells

Esophageal cancer is the top 10 global arising common cancer with limited therapeutic strategies, and the general prognosis is poor owing to diagnosis in late stages in many cases. Sulforaphene (SFE) has been possess anticarcinogenic activities in many cancers, however, it is still not clear how SFE can function as an antineoplastic compound in esophageal cancer. We used microarrays to identify the target genes involved in SFE inhibition of esophageal cancer cell progression. EC109 and KYSE510 cells were cultured and treated with 20 µM SFE or DMSO as negative control for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain the significantly differentially expressed genes between SFE-treated cells and the control group.