Single cell RNA-seq of mouse leptomeninges cells

We performed single-cell RNA sequencing (scRNA-seq) for isolated leptomeninges cells Mouse leptomeninges was manually carefully peeled off from cerebral hemispheres of 3 months old Pdgfra-H2BGFP males and females, digested into single-cell suspensions and subjected to droplet-based ScRNAseq我们对分离得到的软脑膜细胞开展了单细胞RNA测序（single-cell RNA sequencing，scRNA-seq）：从3月龄的Pdgfra-H2BGFP雌雄小鼠大脑半球上手动小心剥离软脑膜组织，将其消化为单细胞悬液后，采用基于液滴的单细胞RNA测序技术进行检测。