The effect of productive HPV16 infection on the cervical epithelium transcriptome

Microarray analysis of the effect of early stage, productive HPV16 infection on the transcriptome of human cervical keratinocytes. Our results provide for the first time, global gene expression changes in cervical epithelium that produces high levels of viral titers. RNA was isolated from 10-day organotypic raft cultures of human cervical keratinicoytes persistently infected with HPV16. RNA isolated from raft cultures of primary cervical keratinocytes were used as control.