A system biology approach reveals neuronal and muscles developmental defects after chronic exposure to ionising radiation

Assessment of the transcriptomics effects of chronic exposure to gamma ionizing radiation (IR) on zebrafish development at dose rates of 0.5mGy/h, 5mGy/h and 50mGy/h. Embryos were exposed chronically to IR from fertlization to developmental stages: 24hpf, 48hpf and 96hpf. Overall design: Chronic exposure of zebrafish fertilized eggs to an external source of gamma radiation (137Cs) at 0.5mGy/h, 5mGy/h and 50mGy/h, plus non-exposed controls. Eggs were exposed continuously to IR at the 3 dos rates until they reach 24hpf, 48hpf and 96hpf stage, and subjected to mRNAseq stranded protocol (Illumina). All conditions were performed in at least triplicated biological experiments.