Effect of pre-miR-1307 overexpression on isomiR levels in MDA-MB-231 breast cancer cells

In the recent past, the impact of isomiR expression changes in cancer has gained increasing interest. Here, we stably overexpress pre-miR-1307, a pre-miRNA which is frequently upregulated in cancer, and assess the effect on isomiR expression. While we see robust and significant upregulation of the canonical form miR-1307-3p|0 and its abundant 5'isomiR miR-1307-3p|1, as well as of the canonical miR from the 5p arm (miR-1307-5p|0), the overexpression of pre-miR-1307 did not lead to global alterations in isomiR expression patterns. These data validate the suitability of our model cell line for the analysis of the effects of joint upregulation of miR-1307-3p|0, -3p|1 and -5p|0. Overall design: MDA-MB-231 breast cancer cells were stably lentivirally transduced with constructs overexpressing pre-miR-1307 or two independent C.elegans pre-miRNA constructs and isomiR expression levels were quantified by small RNA sequencing of three biological replicates per condition.