Impact of Tda1 on gene expression during glycerol cultivation in Saccharomyces cerevisiae. Impact of Tda1 on gene expression during glycerol cultivation in Saccharomyces cerevisiae

The energy metabolism pathways are significantly influenced by the available carbon sources. In Saccharomyces cerevisiae, energy is primarily produced through aerobic respiration during glycerol cultivation, a process believed to depend on the yeast AMPK (AMP-activated protein kinase) homolog, Snf1. It has been reported that Snf1 increases the expression of respiratory genes by phosphorylating transcriptional activators in environments where glucose is unavailable. We discovered that Tda1, activated by Snf1, phosphorylates Hxk2. Hxk2 has been reported to function as a transcriptional repressor. Therefore, we analyzed how Tda1 affects the expression of respiratory genes by RNA-seq analysis of glycerol-cultured cells. Overall design: RNA-seq profiling of BY4741 WT, tda1Δ, snf1Δ, and Hxk2-S15A mutant cells under glycerol culture conditions.