CIL:40123, Caulobacter crescentus NA1000. In Cell Image Library
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Caulobacter crescentus (strain Snc04)were induced for 1 hour at 30 degrees, labeled for 2 hours at 30 degrees (in presence of 1.8 micromolar ReAsH-EDT2); post fixed with _0.01%_ Acrolein and 2.5% of Glut. in 0.01M Sodium cacodylate buffer; subjected to high pressure freezing and freeze substitution by using Epoxy resin as fixative: 20% of the complete Araldite/Epon formulation in acetone. Infiltration and embedding in 100% of resin complex. 0.5um sections were cut and collected on gold grids. Sections were post stained with 4% of Osmium followed by Sato lead and finally 10nm was applied on both sides of sections. Single tilt tomograms were obtained using a JEOL 4000, and spanned -52 - 66° at 2° intervals.
提供机构：
UC San Diego Library Digital Collections
创建时间：
2021-04-15
