Using VBIM technique to discover ARMC4/ODAD2 as a novel negative regulator of NF-?B and a new tumor suppressor in colorectal cancer

Since nuclear factor (NF) ?B plays pivotal roles in in?ammation and cancer, understanding its regulation holds great promise for disease therapy. Using the powerful validation-based inser-tional mutagenesis (VBIM) technique established by us previously, we discovered armadillo re-peat containing protein 4 (ARMC4), a rarely studied protein known to date, as a novel negative regulator of NF-?B in colorectal cancer (CRC). High expression of ARMC4 downregulated the expression of NF-?B-dependent genes. We report the expression differences of NF-kB target genes in a novel inhibitor of NF-kB activity. Through RNA-seq we identified a group of NF-kB target genes specifically regulated by ARMC4. This study provides a framework for identifying genes downregulated and upregulated by a novel inhibitor of NF-kB activity. Overall design: Examination of NF-kB target gene differences in samples with and without Il-1B