Single-nucleus RNA sequencing of gastrocnemius and plantaris muscles.

Skeletal muscle is composed of multinucleated myofibers, in which nuclei share the cytoplasm, and other cell types. Here, we performed single-nucleus RNA sequencing (snRNA-Seq) to determine the gene expressions in each nucleus in gastrocnemius and plantaris muscles collected from 12-week-old C57BL/6 wild-type mice. Our datasets also provided a basis for comparing gene expressions between myofibers and other cell types and discovering the novel cell populations in muscle tissues. Single-nucleus RNA sequencing of gastrocnemius and plantaris muscles of 12-week-old C57BL/6 wild-type mice.骨骼肌由多核肌纤维（multinucleated myofibers）构成，其细胞核共享同一细胞质，同时还包含其他细胞类型。本研究通过单细胞核RNA测序（single-nucleus RNA sequencing，snRNA-seq），对12周龄C57BL/6野生型小鼠的腓肠肌与跖肌中每个细胞核内的基因表达水平进行了检测。本数据集同时为比较肌纤维与其他细胞类型间的基因表达差异、发现肌肉组织中的新型细胞群体提供了研究基础。本数据集涵盖12周龄C57BL/6野生型小鼠腓肠肌与跖肌的单细胞核RNA测序数据。