Fluorescence-Based Nitric Oxide Detection by Ruthenium Porphyrin Fluorophore Complexes

The ruthenium(II) porphyrin fluorophore complexes [Ru(TPP)(CO)(Ds-R)] (TPP = tetraphenylporphinato dianion;
Ds = dansyl; R = imidazole (im), 1, or thiomorpholine (tm), 2) were synthesized and investigated for their ability
to detect nitric oxide (NO) based on fluorescence. The X-ray crystal structures of 1 and 2 were determined. The
Ds-im or Ds-tm ligand coordinates to an axial site of the ruthenium(II) center through a nitrogen or sulfur atom,
respectively. Both exhibit quenched fluorescence when excited at 368 or 345 nm. Displacement of the metal-coordinated fluorophore by NO restores fluorescence within minutes. These observations demonstrate fluorescence-based NO detection using ruthenium porphyrin fluorophore conjugates.