Figure 2 Data

Includes all applicable data pertaining to Figure 2. Bead-bound stimulatory antibodies were used to activate PBMCs (n=3 canine donors) and frequency of activated T cells was determined by cell surface expression of CD25. Different antibody doses were utilized to determine optimal dose for superior T cell activation. The stimulatory effects of four different αCD3+αCD28 bead-bound antibody combinations (1:1 ratio) and two different αCD3+isotype bead-bound antibodies (1:1 ratio) were evaluated: CA17.6F9+5B8 beads, CA17.2A12+5B8 beads, CA17.6F9+1C6 beads, CA17.2A12+1C6 beads, CA17.6F9 beads, and CA17.2A12 beads. Data includes all applicable FCS and Prism files.