Next Generation Sequencing Facilitates Quantitative Analysis of mRNA lifetime of Wild Type and Mettl3-/- mice

Considering m6A modification in mRNA was reported to be intensively correlated with RNA decay, for example, T cells and neurone cells. We wondered whether deleting Mettl3, the main catalzing enzyme, affect the RNA stability of mouse dendritic cells. Overall design: Two sets representing WT and KO dendritic cells were analyzed, each set contains three samples indicating three different treatment with Actinomycotoxin D for 0, 3, or 6h. No replicate was included.