Supplementary File 2. High-throughput miRNA-sequencing of the human placenta: expression throughout gestation

Aim: To understand miRNA changes across gestation in healthy human placentae. This is essential before miRNAs can be used as biomarkers or prognostic indicators during pregnancy. Materials & Methods: Using next-generation sequencing, we characterize the normative human placenta miRNome in first (N=113) and third trimester (N=47). Results and Conclusions: There are 801 miRNAs expressed in both first and third trimester, including 182 with similar expression across gestation (P≥0.05, FC≤2) and 180 significantly different (FDR2). Of placenta-specific miRNA clusters, C14MC decreases across gestation and C19MC is overall highly expressed. Chromosome 13 clusters are upregulated in first trimester. This work provides a rich atlas of healthy pregnancies to direct functional studies investigating the epigenetic differences in first and third trimester placentae.