Transcriptomic profile of circulating memory T cells in donors previously exposed to dengue virus

Dengue virus is responsible for 400 million human infections each year . The characterization of exact molecular components of immune response associated may help design more effective therapeutic interventions. In this study, we aimed to characterize the immune signature of memory T cells associated with previous exposure to dengue virus. Transcriptomic profiling using RNA sequencing was performed on memory CD4 and CD8 T cells isolated from individuals with primary or secondary dengue infections from two endemic areas (Sri Lanka and Nicaragua), as well as from dengue negative healthy controls. Overall design: RNA-sequencing of sorted memory CD4 and CD8 T cells from cryopreserved PBMC of 15 subjects with primary dengue infection, 30 subjects with secondary infection infection and 15 dengue virus negative healthy controls.