Expression profiles of miRNAs in cholestasis mice and normal mice.

We report differences in miRNA expression between cholestasis mice and LIX1L knockout cholestasis mice.Liver samples were obtained from mice from different groups, and the liver samples were detected using the Agilent Mouse miRNA Microarray, Release 21.0 (8*60K,Design ID:070155) chip. After the samples were qualitatively controlled, the data were standardized using the Quantile algorithm. According to the analysis confirmation, a total of three groups of differences were compared and the corresponding GO and KEGG were performed.miR-191-3p displayed the most significant reduction in the liver of WT-BDL mice, and the reduced expression of miR-191-3p was restored in Lix1l-/--BDL mice. We demonstrated that miR-191-3p targets and downregulates LRH-1.Finally, we demonstrated that miR-191-3p further alleviated cholestatic liver injury by inhibiting the expression of LRH-1 to inhibit bile acid synthesis.This study provides a reference for the application of a comprehensive miRNA Microarray to characterize differential miRNA expression in cholestasis mice. Microarray analysis of miRNA expression profiles in cholestasis mice.