m6A sequencing of vitreous bodies of diabetic retinopathy patients

As the most prevalent, abundant and conserved internal cotranscriptional modification in eukaryotic RNAs, N6-methyladenosine (m6A) plays vital role in the progression of different types of human diseases, including cancers. m6A RNA methylation have been reported to affects the physiological and pathological progressions of hematopoietic, central nervous and reproductive systems. As diabetic retinopathy is one of the most importent microvascular complication of diabetes, in this work, MeRIP-seq was employed to investigate the RNA m6A levels in the vitreous body samples of diabetic retinopathy and control groups. A group of mRNAs were suggested with significantly fluctuated transcription and m6A levels, and they would be inverstigated in the further work to understand the mechanisam of the progression of diabetic retinopathy. Overall design: During pars plana vitrectomy, vitreous bodies of diabetic retinopathy patients (DR group, 90 eyes) and non-diabetic retinopathy patients (NC group, 90 eyes, including rhegmatogenous retinal detachment, macular hole, epimacular membrane) were collected. To enrich the RNA, samples of each group were combined (30 samples were mixed) as DR_1-3 and NC_1-3. Comparative gene expression profiling and m6A modification analysis using RNA-Seq data of two groups were performed.