Michael Cammer, Phyllis Novikoff (2011) CIL:35278, Rattus rattus, hepatocyte. CIL. Dataset

Primary rat hepatocytes in culture fixed and stained for beta-tubulin. The microtubules form a network throughout the cytoplasm. In this image they are seen originating from a brighter and denser region adjacent to the cell nucleus.

This is a single optical section a few microns up from the substrate collected by widefield fluorescence microscopy with a Photometrics camera with a KAF1400 chip. The imaging was performed at a microscopy course at Woods Hole Oceanographic Institute in October 1993 and deconvolved on VayTec software running on a Macintosh computer. At the time, deconvolution was new and novel for use by biologists.

培养中的大鼠肝细胞经固定和β-微管蛋白染色。微管在整个细胞质中形成网络。在本图像中，它们从紧邻细胞核的亮度和密度更高的区域起源。这是通过Photometrics相机的KAF1400芯片进行宽场荧光显微镜采集的，距离基底几微米的一个单一光学切片。该成像于1993年10月在伍兹霍尔海洋研究所的显微镜课程上完成，并在Macintosh计算机上运行的VayTec软件中进行了解卷积处理。在当时，解卷积对生物学家而言尚属新颖且具有革命性。