Chromatine immunoprecipation of atonal binding sites in Drosophila L3 eye imaginal discs

Atonal is a proneural transcription factor expressed in the Drosophila eye imaginal discs. To characterize the putative targes of Atonal in the eye discs we have used an endogenously GFP-tagged version of atonal to immunoprecipate disc samples with anti-GFP antibodies Overall design: L3 larvae of the Ato::GFP genotype where dissected to collect the discs. The fixed sample was homogenized by sonication. A 10% of the sonicated volume was processed as input controls while the rest was Immunoprecipitated with antiGFP antibodies, purified and sequenced.