GPCRs detected by proteomic analysis and comparison to those most highly expressed at the transcriptomic level.

List of the GPCRs with the highest transcription level in at least one of the five cell types (expressed as 2−Ct *1012 values >20000) and their identification by proteomic analysis. GPCRs are listed from top to bottom as a function of their mRNA level (the most highly expressed in one or several cell types appears at the top). Number of expected unique peptides corresponds to the unique peptides potentially detectable by MS/MS analysis. Expected peptides were calculated after in silico trypsin digestion of a given GPCR taking into account different criteria: i) no miscleavage, ii) molecular weights comprised between 1000 and 3000 Da, iii) presence in extracellular or cytoplasmic regions and iv) no posttranslational modifications. GPCRs identified by proteomic analysis are indicated in italics. OB1 and TG1 correspond to the GSCs. f-NSC, HA and U-87 stand for fetal neural stem cells, human astrocytes and the U-87 MG GBM cell line, respectively. Ct = cycle threshold; nd = not detected).