Development of gene expression for stress in heat- and shake-induced injury in the rat jejunum

To further development of our gene expression approach to biodosimetry, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish radiation dose across an exposure range relevant for medical decision-making in a radiological emergency. Human peripheral blood from healthy donors was irradiated ex vivo, and a 74-gene consensus signature was identified that distinguished between four radiation doses (0.5, 2, 5 and 8 Gy) and control samples. The same set of genes separated samples by exposure level at both six and 24 hours after treatment, with overlap evident only at the highest two doses (5 and 8 Gy). Expression of five genes (CDKN1A, FDXR, SESN1, BBC3 and PHPT1) from this signature was quantified in the same RNA samples by real-time PCR, confirming low variability between donors as well as the predicted radiation response pattern. Heat and shake stress induced gene expression in rat jejunum was measured with or without 3 days stressed (Control and S3d groups) The conditions of the treatment for rats were as follows: 35°C with a vibration of 0.1 × g for 3 days， 2 h/day in a temperature shake. each group had 3 sample replications, and each sample were mixed by 3 rats total RNA