Expression analysis of tomato S. pimpinellifolium accession LA1589 wild type and the Nearly Isogenic Lines lc, fas and lc/fas NILs during floral development.

The goal of this study is to decipher the effect of the tomato lc and fas mutation on gene expression during floral development in tomato. The 3'Tag RNA libraries were sequenced on a Illumina HiSeq2500 at Genomic Resources Core Facility at Weill Cornell Medical College for 100 bp single–end sequencing. Total raw reads for each sample ranged between 5 to 10 million per sample. For each genotype, samples from 5-7 week old seedlings were collected in triplicate in the greenhouse and immediately stored in RNAlater. Using the dissecting microscope, sympodial shoot apical meristem (SYM), the combined floral and inflorescence meristem (F&IM), and floral buds at 2, 4, and 6 days post floral initiation (dpi) were collected and frozen in the -80 C.