Gene.ann.tpm.tsv

Gene annotation was primarily performed by using KofamKOALA. Unannotated genes would be aligned with Uniprot database by DIAMOND. OGs were determined by eggnog-mapper. Raw reads were first tirmmed by Trimmomatic for poor-quality base and then filtered by Ribodetectorfor remove rRNA reads. The unaligned mRNA reads were mapped to the representative MAGs by BBmap . Finally, featureCounts was used to count the read number of each gene.