Single cell RNA sequencing of murine gamma delta T cells from wild type and beta2 integrin-deficient (CD18 KO) lungs

The beta2 integrin subunit (CD18) is thought to regulate gamma delta T cells in vivo. The objective of this study was to determine the gene expression changes mediated by loss of CD18 in lung gamma delta T cells. Single cell RNA sequencing was performed on gamma delta T cells (CD3+ gamma delta TCR+) isolated from the lungs of wild type C57BL/6 mice (N=2) and CD18 knockout (Itgb2-/-) mice (N=1).β2整合素亚基（beta2 integrin subunit）CD18被认为在体内调控γδ T细胞（gamma delta T cells）。本研究旨在明确肺脏γδ T细胞中CD18缺失所介导的基因表达变化。研究人员对从野生型C57BL/6小鼠（N=2）与CD18敲除（Itgb2-/-）小鼠（N=1）肺脏中分离得到的γδ T细胞（CD3+ γδ TCR+）开展了单细胞RNA测序（single cell RNA sequencing）。