Mus musculus Raw sequence reads

It is well established that the histone chaperone FACT promotes chromatin recovery during transcription. To address the role of FACT regulates genome wide chromatin accessibility and its impact on transcription factor binding. We deleted the FACT component Ssrp1 in MEF cells. We show here that Ssrp1 is required for normal progression of cell phase transition, cell proliferation, and DNA damage repair in mouse embryonic fibroblast cells. Intriguingly, depletion of the Ssrp1 impairs normal chromatin structure in mouse zygotes and compromises early embryonic development. On the molecular level, the absence of the Ssrp1 leads to a dramatic increase in genome-wide chromosome accessibility, enhanced CTCF binding, and a remarkable decrease in a dynamic range of gene expression. Our study thus unequivocally uncovers a unique mechanism by which FACT complex regulates transcription by balancing genome wide chromatin accessibility and CTCF binding.