High-Throughput Sequencing of the 5S-IGS nuclear DNA in Viola sect- Melanium (Violaceae)

This fileset reports intra-genomic, intra- and inter-individual variation of the multycopy, nuclear encoded 5S rDNA region in four Viola (V. tricolor, V. arvensis, V. kitaibeliana, V. hymettia) and outgroup species of section Melanium. The gathered data were obtained with Pair-end Illumina sequencing (2x300 bp).The Fileset includes:Dataset File S1 = the sample list with details about sampling, taxonomy, vouchers, ID, growing habitats;Dataset File S2 = a description of the working dataset, including pre-processing steps, sample distribution in each sample, sequence structural features.Also included are the multiple alignments (FASTA format) of the total (sequence abundance >5) and a filtered (989 sequences with abundance >25 and length >280 bp) dataset, and the derived phylogenetic tree (TREES format).