Detection and differentiation of Clostridium botulinum strains. Detection and differentiation of Clostridium botulinum strains

Strains were differentiated on the basis of hybridization to probes representing strain variable regions in C. botulinum strain ATCC 3502. Probes for selected genes (eg. toxin genes) were also featured on the microarray to allow detection of other serotypes/subtypes. Overall design: 44 strains were evaluated for selected gene detection and/or strain differentiation. DNA from strain ATCC 3502 was used as a control as the featured probes were based on the ATCC 3502 genome sequence.