Identification of genes regulated by the MADS transcription factor (TF), SEPALLATA3 spliced variant, SEP3.3 , in the context of the triple sep1 sep2 sep3 mutant background, by RNA-Seq analysis

These analyses allowed the identification of more than 2000 genes regulated by the MADS TF, SEPALLATA3.3 spliced variant(renamed SEP3?tet or SEP3deltet). Overall design: RNA seq analysis were performed on inflorescence meristem and flower buds up to stage 10-11 harvested from Arabidopsis plants grown in parralel in long days conditions. Experiments were performed on 3 independant lines sep1sep2sep3 expressing SEP3?tet . These lines are described in Hugouvieux et al, Nucleic Acid Research, 2018:1;46(10):4966-4977. doi: 10.1093/nar/gky205. Libraries and sequencing were performed by Genewiz (Illumina High SEq, 2X150 bp configuration).