Transcription profiling of HNF4 alpha null mice to investigate the phenotype of dis-regulated insulin secretion and abnormal beta cell growth

The aim of this experiment was to use microarray analysis to examine the phenotype of dis-regulated insulin secretion and abnormal beta cell growth in HNF4 alpha null mice. These mice show impaired glucose tolerance and elevated fasting and fed plasma insulin levels. Rana Gupta from Klaus Kaestner's Lab extracted RNA from isolated islets. Three controls and five mutants were provided for the study.本实验旨在通过基因芯片（microarray）分析，探究HNF4α敲除小鼠（HNF4 alpha null mice）中胰岛素分泌失调与胰岛β细胞异常生长的表型。 该类小鼠表现出糖耐量受损，且空腹及餐后血浆胰岛素水平升高。 来自克劳斯·凯斯特纳实验室（Klaus Kaestner's Lab）的拉娜·古普塔从分离的胰岛中提取了核糖核酸（RNA）。 本研究共纳入3例对照样本与5例突变样本。