Kinetic constants for gluconeogenic and anaplerotic reactions catalyzed by wt and F502A; F510A; F515A; F502, 510A; F510, 515A;F502, 515A Pck mutants.

aFor gluconeogenic reaction: 100 mM HEPES-NaOH, pH 7.2, 0.3 mM OAA, 2 mM MgCl2, 0.2 mM MnCl2, 10 mM DTT, 10 U/ml LDH, 3 U/ml PK and 0.2 mM NADH was used. bFor anaplerotic reaction: 100 mM HEPES-NaOH, pH 7.2, 100 mM KHCO3, 37 mM DTT, 2 mM PEP, 2 mM MgCl2, 0.1 mM MnCl2, 2 U/ml MDH and 0.25 mM NADH was used. n.d.: Kinetic constants cannot be calculated. Kinetic constants for gluconeogenic and anaplerotic reactions catalyzed by wt and F502A; F510A; F515A; F502, 510A; F510, 515A;F502, 515A Pck mutants.