Histological evaluations of mice with or without EAC-induced cystitis after control and/or anti-CXCL10 Abs treatment.

At the end of experiment the severity of urinary bladder inflammation was evaluated by histopathological changes described in the methods section for EAC induction and treatment. Mice were sacrificed at the experimental end point and urinary bladders was fixed in 10% neutral formalin for 24 hrs and embedded in paraffin. Fixed tissues were sectioned at 6 µm, stained with hematoxylin and eosin, and examined by light microscopy. The inflammatory state of each urinary bladder was characterized and scored as described in the methods section. The studies were repeated 3 times, and the data presented are the mean score changes ± SEM of these experiments. Differences between anti-CXCL10 Abs treated and EAC induced control Abs treated group were considered significant when p<0.01 (ζ).