Figure S1 - The Astrocyte-Targeted Therapy by Bushi for the Neuropathic Pain in Mice

Suppression of microglial activation by minocycline at the erarly induction phase resulted in inhibition of subsequent astrocytic activation at the late maintenance phase. Immunohistochemical analysis of microglia (Iba-1-positive signals) (A, B) and astrocytes (GFAP-positive signals) (C, D), showing effect of chronic administration of minocycline (30 mg/kg, p.i.). The administration protocol was same as Fig. 4A (from −d7 to d14). Rectangulars in A and C were expanded and shown as high magnification images in the corresponding lower pannels. Suppression of microglial activation (A) resulted in inhibition of subsequent astrocytic activation at the late maintenance phase (E; SNL-Minocycline (d14/m21), Ipsi), which was summarized in B (microglia) and D (astrocytes). $$p<0.01 versus Sham-Vehicle Contra. †† <0.01 versus Sham-Vehicle Ipsi. Scale bars: 50 µm.
(EPS)