Spatial transcriptomics of P35 septum

Purpose: The goal of this study is to use MERFISH to identify the spatial distribution of septal cells Methods: P35 CD1 mice were collected.10 µm tissue sections were obtained from along the rostro-caudal axis and adhered to the MERSCOPE Slide. The samples were permeabilized in 70% ethanol overnight, followed by cell boundary staining. A 500-gene panel was generated based on enriched genes in each astrocyte and neuron cluster, with approximately 100 astrocyte genes and 400 neuron genes, The samples were then hybridized using the gene panel mix, followed by gel embedding and clearing steps. Images were processed using the MERSCOPE instrument and analysis computer, along with MERSCOPE visualizer software to streamline the acquisition of high-quality MERFISH data accroding to Vizgen; Results: Neurons and astrocytespatial clusters were defined in the septum based on unique gene enrichment Defining septal neurons and astrocytes spatial clusters in P35 septum