Identifying Ipr1 binding sites in RAW264.7 cells using ChIP-seq

It has been demonstrated that Ipr1 participate in gene expression regulation during Mycobatrium infection. To better understand whether Ipr1 can regulate gene expression by interacting with gene promoter directly or not, we performed ChIP-seq to identify interaction between Ipr1 and DNA fragments. Raw-Bap-Ipr1-P2A-BirA and Raw-Bap-P2A-BirA (negative control) cells were used for ChIP-seq, Flag (FlagChIP bond) and biotin (BioChIP bond) tags were used to isolate Ipr1-DNA complexes.