<i>B. nonstop</i> annotated proteins

Protein sequences from <i>B. nonstop</i> were additionally annotated by blastp searches (with a cut-off <i>e</i>-value of 10^-20) against protein datasets of a selection of kinetoplastids available from TriTrypDB (release 62) including <i>Angomonas deanei</i>, <i>Blechomonas ayalai</i>, <i>Bodo saltans</i>, <i>Crithidia fasciculata</i>, <i>Endotrypanum monterogeii</i>, <i>Leishmania major</i> Friedlin, <i>Leishmania martiniquensis</i>, <i>Leishmania mexicana</i>, <i>Leptomonas pyrrhocoris</i>, <i>Leptomonas seymouri</i>, <i>Paratrypanosoma confusum</i>, <i>Porcisia hertigi</i>, <i>Trypanosoma brucei</i> TREU927, <i>Trypanosoma cruzi</i> CL Brener Esmeraldo-like. Proteins not retrieving any hit were then screened (with a cut-off <i>e</i>-value of 10^-5) against the Swiss-Prot database (downloaded February 10^th, 2023) (Bairoch &amp; Apweiler 2000, Nucleic Acids Res). Protein domains were further annotated using InterProScan v5.55-88.0 (Jones <i>et al.</i> 2014, Bioinformatics) and the Pfam database (Finn et al. 2014, Nucleic Acid Res).来自*B. nonstop*的蛋白质序列通过BLASTp搜索完成了额外注释，比对筛选阈值设置为E值（E-value）≤10⁻²⁰，比对数据库为从TriTrypDB数据库第62版获取的精选动质体生物蛋白数据集，涵盖*Angomonas deanei*、*Blechomonas ayalai*、*Bodo saltans*、*Crithidia fasciculata*、*Endotrypanum monterogeii*、*Leishmania major* Friedlin、*Leishmania martiniquensis*、*Leishmania mexicana*、*Leptomonas pyrrhocoris*、*Leptomonas seymouri*、*Paratrypanosoma confusum*、*Porcisia hertigi*、*Trypanosoma brucei* TREU927以及*Trypanosoma cruzi* CL Brener Esmeraldo-like等物种。对于未检索到任何同源比对结果的蛋白质，随后以E值≤10⁻⁵为阈值，针对Swiss-Prot数据库（2023年2月10日下载，Bairoch & Apweiler, 2000, *Nucleic Acids Research*）开展比对筛选。此外，使用InterProScan v5.55-88.0（Jones等, 2014, *Bioinformatics*）与Pfam数据库（Finn等, 2014, *Nucleic Acid Research*）对蛋白质结构域进行了进一步注释。