Gene expression in GLN versus PDLN in NOD mice

Comparison of the gene expression in the two pancreatic lymph nodes: gastric lymph node (GLN) and pancreatico-duodenal lymph node (PDLN) in NOD mice. The analysis was done in an attempt to explain the preferential homing of bone marrow-derived dendritic cells to the GLN after intravenous injection. GLN and PDLN were excised from 4 individual 12-wk old female NOD mice and RNA was extracted after tissue homogenization in Trizol for processing onto microarray (Agilent 4x44K). Each array corresponds to one single mouse, thus this study comprise 4 biological replicates.本研究对非肥胖糖尿病（NOD）小鼠的两种胰腺淋巴结——胃淋巴结（GLN）与胰十二指肠淋巴结（PDLN）的基因表达水平开展对比分析，旨在阐释经静脉注射后骨髓源性树突状细胞（bone marrow-derived dendritic cells）优先归巢至胃淋巴结的现象。研究人员从4只12周龄雌性非肥胖糖尿病小鼠体内分别摘取上述两种淋巴结，经Trizol试剂完成组织匀浆后提取RNA，用于Agilent 4x44K基因芯片检测。每张芯片对应单只小鼠，因此本研究共包含4个生物学重复样本。