High-throughput sequencing of Drosophila melanogaster small RNAs. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Keywords: high-throughput 454 s

NIAID Data Ecosystem20

Mapping of dsRNA in yeast using reconstituted RNAi pathway. Saccharomyces cerevisiae

小RNA测序

RNA干扰通路分析

Small RNA produced by Dicer (Dcr1) are used to map dsRNA in wild-type strain and a xrn1-delta mutant of S. cerevisiae, inactivated for the cytoplasmic 5'-3' RNA decay pathway. Overall design: Small RN

NIAID Data Ecosystem40

Neurospora crassa small RNA sequencing of sexual development timepoints. Neurospora crassa OR74A strain:FGSC 2489

减数分裂沉默

小RNA测序

N. crassa small RNA sequencing of meiotic tissue to study unpaired DNA silencing response. Small RNA from meiotic tissue of sexual cross between N.crassa strains FGSC 2489 and FGSC 8820.

NIAID Data Ecosystem20

High throughput sequencing of endogenous small RNAs from AGO pathway mutants

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