Defining the microglial transcriptome in the rat subventricular zone (SVZ) after neonatal hypoxia-ischemia (HI).

Purpose: Recent findings indicate a regulatory role of microglia on neurogenesis in the SVZ in health and disease. Microglia in the early postnatal SVZ are not fully maturated and may react differently than adult microglia to injury. We sought to investigate the impact of cortico-striatal neonatal HI injury on the microglial phenotype in the early postnatal SVZ. Results: In comparison to sham SVZ microglia, HI SVZ microglia upregulate both pro- and anti-inflammatory genes as well as neurotrophic genes. HI SVZ microglia do not adopt a M1 or M2 polarization state, but instead share commonly expressed genes with microglia in rodent models of neurodegenerative diseases.研究目的：近期研究表明，小胶质细胞（microglia）对健康与疾病状态下室下区（subventricular zone, SVZ）的神经发生具有调控作用。出生后早期的室下区小胶质细胞尚未完全成熟，其对损伤的应答反应与成年小胶质细胞存在差异。本研究旨在探讨皮质纹状体新生儿缺氧缺血（hypoxic-ischemic, HI）脑损伤对出生后早期室下区小胶质细胞表型的影响。 研究结果：与假手术组室下区小胶质细胞相比，缺氧缺血组室下区小胶质细胞可上调促炎基因、抗炎基因及神经营养基因的表达。该组室下区小胶质细胞并未呈现典型的M1或M2极化状态，而是与啮齿类神经退行性疾病模型中的小胶质细胞共享共有表达基因。