Sensitivity of AUC0–7 for an antibody in mice to changes in Vv, Vi and Q.

Note: Comparison of model-predicted [13] and experimentally measured AUCs [%ID/g day], up to 7 days post-injection, calculated for an intact antibody in mice. All blood flow rates (Q), vascular (Vv) and interstitial (Vi) volumes were simultaneously changed from literature to measured values (see Tables 1, 2, 3) for these simulations, with the exception of Vi for kidneys, for which literature values were always used due to the effects of probe clearance. Measurement of Vv was accomplished using a previously reported indirect RBC labeling method [2].aData for 125I-trastuzumab; reproduced with permissions [2].