Primordial cardiomyocytes orchestrate myocardial morphogenesis and vascularization but are dispensable for regeneration

The vertebrate heart is composed of heterogeneous cardiomyocyte (CM) populations, however, the roles of distinct CM subpopulations in heart development and repair remain poorly defined. Here, using single-cell RNA sequencing analysis of adult zebrafish heart, we identified a unique CM subpopulation marked by the expression of phlda2, which is associated with anaerobic metabolism and different from mature CMs which are enriched for oxidative phosphorylation genes. We demonstrated that phlda2+ cells constituted a primordial CM compartment localized between compact and trabecular muscles. Genetic ablation of phlda2+ CMs during development severely disrupted heart morphogenesis, leading to defective myocardial trabeculation and compaction, and impaired coronary vascularization. Surprisingly, despite their essential roles in development, the depletion of phlda2+ CMs didn't impair myocardial restoration and revascularization following ventricular resection. We found that this was probably due to the limited regenerative capacity of the primordial CMs themselves, as they failed to regenerate after either surgical amputation or genetic ablation. Our findings identify primordial CMs as an organizer for heart morphogenesis but not essential for regeneration, revealing a fundamental difference between developmental and regenerative programs in the vertebrate heart. Overall design: Single cell RNA sequencing of FACS isolated cardiomycytes (cmlc2:EGFP) from adult hearts