Transcritpomic analyses of CARD14 WT and psoriatic gain-of-function CARD14 E138A mutant over time

CARD14 gene mutations have been shown to be a causitive factor for psoriasis in human patients. CARD14 signalling is poorly understood and very little is known about how it regulates NF-kB activation. Basic research is therefore required to better understand the mechanisms by which is brings about pathology in a gain-of-function state. Analyses of CARD14 mutant versus WT transcription over time in a relevant cell type (keratinocyte) was pursued as one of several means to better understand CARD14-driven psoriasis. Overall design: CARD14E138A-3xFLAG HaCaT-TR, CARD14WT-3XFLAG HaCaT-TR keratinocytes were seeded for tetracycline incubations of 0, 3, 6, and 9 h. Cells were lysed and RNA isolated (RNeasy kit, QIAGEN, #74106). mRNA was then purified using a poly-A KAPA mRNA Hyper Prep kit (Roche) and sequenced on an Illumina HiSeq4000 instrument.