S3 Table. All the raw data for quantitative PCR -
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2020-10-01
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Log2 fold changes of the four specifically amplified genes for validation resulting from the MACE analysis and RT-qPCR (calculated using REST with correction of amplification efficiencies).
SE = standard error.Log2 fold changes of the four specifically amplified genes for validation resulting from the MACE analysis and RT-qPCR (calculated using REST with correction of amplification effic
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RNAseq is not required to determine stable reference genes for qPCR normalization
The original contributions presented in the study are included in the article & Supplementary Data. The metadata for the RNA-Seq and qPCR include the normalized count matrices for the RNAseq data,
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Analysis outputs of the workflows for RNA-seq data analysis.
Analysis outputs of the workflows for RNA-seq data analysis.
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MOESM1 of Combined quantitation of HMGA2 mRNA, microRNAs, and mitochondrial-DNA content enables the identification and typing of thyroid tumors in fine-needle aspiration smears
Additional file 1: Table S1. The oligonucleotide sequences used in the study.
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R code for the MCTOT shiny application.
As a common experimental technique, qPCR (Quantitative Real-time Polymerase Chain Reaction) is widely used to measure levels of nucleic acids, e.g., microRNAs and messenger RNA. While providing accura
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