Dynamics of the CD9 interactome during bacterial infection of epithelial cells by proximity labelling proteomics

Bacterial species utilise different receptors at the cell membrane to adhere to cells. Previously, we demonstrated that interference with CD9, a human tetraspanin, reduces adherence of multiple species of bacteria to cells. CD9 is not a receptor but organises numerous commandeered host proteins at the cell membrane; however, the full interactome has not yet been delineated. Using a CD9 proximity labelling model, a first for CD9, we observed a diverse interactome, with 710 enriched proteins in uninfected cells. Proximal proteins were associated with various cellular processes, including extracellular matrix (ECM)–receptor interactions and tight junctions. Several known bacterial receptors were also detected, including CD44, CD46, and CD147. The interactome was dynamic during infection with two distinct bacterial species, Neisseria meningitidis and Staphylococcus aureus. In total, 12 human proteins were enriched during meningococcal infection, compared to one during staphylococcal infecti..., , # Data from: Dynamics of the CD9 interactome during bacterial infection of epithelial cells by proximity labelling proteomics

Dataset DOI: [10.5061/dryad.m905qfvfc](10.5061/dryad.m905qfvfc)

## Description of the data and file structure

**Data S2. Cell compartment and cellular pathway analysis of identified proteins.** Data files contain analysis of significantly enriched proteins identified by mass spectrometry at 30, 60 and 240 minutes. Data files supplied by SubcellulaRVis demonstrates the cellular compartment analysis of the enriched proteins. Data files supplied by WebGestalt provide the KEGG pathway analysis of the significantly enriched proteins.

### Files and variables

#### File: CellVis_Enrichment_30_mins.csv

**Description:** Cellular compartment analysis from SubcellulaRVis after 30 mins.

#### File: CellVis_Enrichment_60_mins.csv

**Description:** Cellular compartment analysis from SubcellulaRVis after 60 mins.

#### File: CellVis_Enrichment_240_mins.csv

**Description:*...,